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DOT Pre-Employment Drug Test Requirements

Written by Nick Webster

Published on Dec. 11, 2023, 2:35 p.m.

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Are you ready for a new job that involves DOT pre-employment drug test requirements? Before you can land that dream position, there is one critical step to take: passing the DOT (Department of Transportation) pre-employment drug test. This critical requirement ensures that those in safety-sensitive positions remain free from drugs and alcohol while on the job. Visit The DOT Physical Drug Test.








Written below is what you need to know before testing day. If you have any questions, do not hesitate to contact our organization. Also, keep in mind that FMCSA (Federal Motor Carrier Safety Administration) regulations refer to DOT requirements. Must visit the link.



What Is FMCSA Pre-Employment Drug Testing?



FMCSA pre-employment drug testing is a critical part of the hiring process for commercial truck drivers and other transportation industry employees. It ensures that all new staff members are drug-free and can provide safe, reliable service to customers. The FMCSA and DOT require employers in the trucking and transportation industries to screen all potential hires before they are allowed to begin work. This includes providing confirmation of a negative result from an authorized laboratory or collection site for a pre-employment drug test. What Is DOT Drug Testing?


When a potential hire provides their sample, it is tested for several illegal drugs, including:


  • marijuana
  • cocaine
  • opiates
  • amphetamines
  • PCP
  • barbiturates
  • methadone
  • benzodiazepines



How Does a Business Carry Out FMCSA Pre-Employment Drug Testing?



The FMCSA has established regulations for employers to ensure that all truck and bus drivers are safe and responsible on the road. As part of those regulations, employers must carry out pre-employment drug testing. Here are the steps that a business must take to comply with FMCSA pre-employment drug testing requirements:


  • Businesses must use a Substance Abuse Professional (SAP), such as those certified by the Department of Transportation, to design and oversee their drug testing program.
  • SAPs will help businesses develop sound policies and procedures that are compliant with FMCSA regulations.
  • They will also ensure that any results are safely stored, reported accurately, and promptly acted upon in accordance with federal regulations.
  • Most pre-employment drug tests must be conducted using an approved laboratory or a Medical Review Officer (MRO).
  • MROs can contact employers if they have questions about a potential employee’s drug test result.
  • Employers should use physical examinations administered by licensed healthcare professionals.


To begin the process of drug testing, an employer must first register with the Drug & Alcohol Testing Clearinghouse and provide information about each driver who will be tested. Once registered, the employer can designate a Drug Test Collection Facility that is approved by the Department of Transportation (DOT). This facility will be where you send your newly hired employees for their pre-employment drug testing. Visit How Much Does a DOT Number Cost?



When Do You Not Have to Do FMCSA Pre-Employment Drug Testing?



FMCSA pre-employment drug testing is one of the core requirements for safety-sensitive truck drivers. However, there are certain situations when it may not be necessary. For instance, if a driver has been regularly tested and maintained satisfactory results in the two years prior to employment with a new carrier, then FMCSA drug testing is not required.

Also, drivers who were employed within the last 30 days by another motor carrier and subject to regular drug tests are also exempt from FMCSA pre-employment testing. In both these cases, employers should document this information to ensure compliance with FMCSA regulations. It is important to note that employers remain responsible for ensuring their employees have undergone adequate drug screening prior to beginning work as a commercial vehicle driver. Read about USDOT Number. Learn here How to Prepare for a Compliance DOT Audit.





Is There an FMCSA Pre-Employment Alcohol Test?



The FMCSA does not require employers to conduct pre-employment alcohol tests for drivers. However, FMCSA regulations do state that drivers must be tested for alcohol if they are involved in an on-duty crash, and the employer has reasonable grounds to believe the driver was impaired. The FMCSA also mandates post-accident testing when there is a fatality or a disabling injury of anyone other than the driver who caused the accident. Visit Driver Qualification Files.

Even though FMSCA does not require direct alcohol testing, employers may choose to include drug and/or alcohol tests as part of their pre-employment screening process if they wish. This type of testing can help ensure that only qualified, safe drivers operate across America’s highways.



Do DOT Pre-Employment Screenings Check For Past DOT Violations?



Yes, DOT pre-employment screenings do check for past DOT violations! This is to ensure that any potential candidate has a clean driving record and can be trusted to drive professionally while on the job. Employers will review an applicant's motor vehicle record (MVR) to determine if they have had any violations in the past related to their work as a driver, such as speeding tickets or DUI convictions. This helps employers determine if a person is fit for the role, and can help ensure that only drivers with a safe history are hired. With this information, employers can make sure their drivers are prepared and qualified to do their job safely and efficiently.



Is the FMCSA Clearinghouse Used for Pre-Employment Drug Testing of Drivers?



Yes, the FMCSA Clearinghouse is used for pre-employment drug testing of drivers. Employers who are subject to FMCSA regulations must conduct a query of the Clearinghouse prior to hiring or offering employment to any driver who has been tested and certified under Part 382 of the Federal Motor Carrier Safety Regulations (FMCSRs). You will need driver qualification files filed out by all employees who drive for you working.

The employer must register with the Clearinghouse in order to perform queries. The results will indicate whether or not the driver has ever tested positive on an alcohol or controlled substances test. Employers must use the information obtained from their queries to determine if they will hire a driver and, if so, what further action they may need to take. Also, take a look at the Drug and Alcohol Program policy. What is DOT Clearinghouse Enforcement?



How Do I Register with the FMCSA Clearinghouse?



Suppose you're an owner-operator of a commercial motor vehicle or employer subject to the drug and alcohol program requirements set forth by the FMCSA. In that case, you need to register with their Clearinghouse. But don't worry, registering is easy! All you have to do is:


  • create an account on the FMCSA website
  • provide the required information
  • pay any applicable fees

Once registered, employers can query prospective and current employees for violations of FMCSA's drug and alcohol rules, while owners-operators can verify themselves as compliant with those same rules. Registering with the FMCSA Clearinghouse allows employers and drivers to comply with the government.



Does a DOT Urine Test Check for Drugs?



Yes, a DOT urine test will check for drugs! Here's the breakdown of what they'll be testing for:

  • Cocaine
  • Marijuana
  • Opiates (including heroin)
  • Amphetamines (including methamphetamine, MDMA and MDA)
  • Phencyclidine (PCP)
  • Benzodiazepines
  • Barbiturates

So, if you're looking to pass a DOT drug test, make sure to stay away from these substances! It's not worth the consequences of failing one of these tests! Good luck!


What Is 49 CFR Part 40?



49 CFR Part 40 is an important part of the FMCSA regulatory framework for drug and alcohol testing. The regulation outlines the requirements for employers to test their employees for controlled substances and procedures for conducting workplace drug and alcohol tests. It also includes information on compliance with DOT regulations and penalties.

This regulation has been in effect since 1988 and must be followed by any business or organization that operates a motor vehicle involved in interstate commerce. So if you're a person who wants to follow DOT regulations, 49 CFR Part 40 is crucial for you to read!


Who Must Receive DOT Drug Tests?


  • Employees in safety-sensitive roles that require the use of a commercial motor vehicle (CMV). This includes school bus drivers, truck drivers, and other positions as required by the DOT.
  • Employees who are subject to random testing due to previous drug abuse or at-risk behavior.
  • Drivers involved in an accident involving a CMV where any one person is killed or injured requiring medical attention away from the scene of the accident.
  • Drivers suspected of being under the influence while on duty.
  • Drivers returning to work after completing a rehabilitation program for substance abuse.
  • Drivers are required by their employers to submit to DOT drug tests. Must visit the link.


What Happens After Failing the DOT Drug Test?



If an individual fails a DOT drug test, employers must immediately remove the employee from performing any safety-sensitive functions. The employer may also be required to report results to the FMCSA. Depending on the jurisdiction, employers may be obliged to report failed tests and implement a rehabilitation process. Failure to comply with applicable regulations may result in significant fines or other penalties. Read about Texas DOT Numbers and USDOT Numbers. Learn here How to Prepare for a Compliance DOT Audit.

Employees who fail their DOT drug test will typically need to go through a return-to-duty process before they can resume safety-sensitive duties. This includes completing an evaluation by a Substance Abuse Professional (SAP) and then enrolling in treatment as prescribed by the SAP.



Has the DOT Updated Its Drug Testing Policies in Recent Years?


Yes, the Department of Transportation updated its drug testing policies in recent years. The DOT instituted new regulations on October 1, 2020, that included changes to how drug tests are administered and reported by employers. The regulations also include a new requirement for employers to test employees for marijuana-based drugs and synthetic cannabinoids randomly. What is DOT SAP Program?

Also, there is now an increased emphasis on safety-sensitive employees being tested through the National Drug and Alcohol Clearinghouse. Finally, the DOT requires all employers to train their supervisors on recognizing signs of employee impairment resulting from substance misuse or abuse. These changes are meant to enhance the safety of our nation’s roads and highways while keeping truck drivers safe from drug-related incidents. What are the Top 3 DOT paper-appointedViolations?



Summary: The Requirements of DOT Pre-Employment Drug Tests



Here is a summary of the information provided on this web page. If you need more info about DOT pre-employment drug tests, do not hesitate to reach out to our third-party organization. We’re prepared to help you and your carrier adhere to all DOT and FMCSA pre-employment regulations. This way, you can maintain compliance with the government at all times.


  • Prospective employers must follow DOT regulations when conducting pre-employment drug tests.
  • Tests must be conducted using a laboratory certified by the Substance Abuse and Mental Health Services Administration (SAMHSA).
  • Test results must be reviewed by a Medical Review Officer (MRO) who is licensed in the state where the test was conducted.
  • Employers must also inform job applicants about their rights and responsibilities under DOT drug testing rules.
  • All specimens must be collected following appropriate protocols, including chain of custody requirements.
  • Federal law prohibits employers from discriminating against those who hold certain types of medical marijuana cards or are legally prescribed certain controlled substances.
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